The ZeeWee™ I Bird DNA Sex Test is an in-house DNA test to determine chickens’ sex (Gallus gallus domesticus). Do not count your chickens before they hatch, PCR them!
This is a test for real chickens, using blood which you will need to collect, or have a vet collect for you. Alternatively, feather follicles may be used.
ZeeWee™ I Bird DNA Sex Test is an in-house DNA test for the determination of sex in chickens (Gallus gallus domesticus). No need to ship samples to a lab, obtain results in hours!
-
Sample collection is easy: DNA is extracted from dried blood spots and feather follicle samples. Result interpretation is clear: two bands indicate a female, one band a male.
This is a gelPCR™ test, based on PCR and DNA gel electrophoresis.
One kit of ZeeWee™ I Bird DNA Sex Test provides enough material for 32 tests and 6 controls (38 reactions). The kit includes reagents and PCR tubes.
How it works:
- Setup and lysis (30 min)
- PCR (85 min)
- Electrophoresis (45 min)
Notes:
- One kit provides enough reagent for 32 tests and 6 controls, plus ten strips of 8 PCR tubes
- This kit requires a thermal cycler (mini8 or mini16 depending upon your requirements) and an electrophoresis unit (the reagents are optimised for blueGel)
- You will also require micropipettes - 0.5-10uL micropipette and tips, 2-20uL micropipette, 20-200uL micropipette and tips (2-20uL and 20-200uL micropipettes use the same tips) and 100-1000uL micropipette and tips.
- For electrophoresis you will require TBE, agarose and Midori green fluorescent stain. These are also available in a reagent kit.
- You will also need microcentrifuge tubes
- Optional but recommended items - tube rack, PCR rack and microcentrifuge.
- See user guide below pg 5-6 for other laboratory supplies required
