Description: TBE Buffer 20x Concentrate.
Preparing TBE buffer:
MC44.12 TBE Buffer Solution for gel electrophoresis of DNA is a 20X concentrate of 100mL volume that should be diluted to produce a working buffer solution. To prepare your 1X working solution, redissolve any precipitated salts that may be present by warming the concentrate, then dilute the 20X concentrate by a factor of 20.
For example,
Vol of 20X Concentrate | Volume of Distilled Water | Volume of Working Buffer (1X conc) |
25mL | 475mL | 500mL |
50mL | 950mL | 1,000mL |
100mL | 1,900mL | 2,000mL |