Investigate how DNA sequence, temperature, and pH interact with the double helix!
Discover the conditions that make double-stranded DNA denature into single strands, then anneal back together again. In this lab, students will use a fluorescent dye to investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. Inquiry-based investigations explore how DNA sequence, concentration, and pH affect base pairing through hydrogen bonds.
Key info and benefits:
- Techniques: Micropipetting, fluorescence detection
- Time required: 45 minutes
- Level: High school
- The DNA Glow Lab kit contains reagents for 8 lab groups of up to 4 students each (32 students).
- DNA samples for analysis
- DNA binding dyes
- Glow Lab Dilution Buffers
- See resources below to download the teacher's guide and student guide for this lab. Contact Southern Biological for answers key.
- A thermal cycler is advised for the heating steps, available as a mini 8 or mini 16 machine.
- The fluorescence can be detected on a blueGel electrophoresis apparatus, a blue box transilluminator or a molecular fluorescence viewer,
- These DNA samples require refrigerated storage (4 degrees), which will keep them stable for 6 months. They are stable for a couple of weeks at room temperature.