Investigate how DNA sequence, temperature, and pH interact with the double helix!
Discover the conditions that make double-stranded DNA denature into single strands, then anneal back together again. In this lab, students will use a fluorescent dye to investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. Inquiry-based investigations explore how DNA sequence, concentration, and pH affect base pairing through hydrogen bonds.
Key info and benefits:
- Techniques: Micropipetting, fluorescence detection
- Time required: 45 minutes
- Level: High school
- The DNA Glow Lab kit contains reagents for 8 lab groups of up to 4 students each (32 students). These reagents are compatible with blueGel™ or other standard DNA electrophoresis systems.
- DNA samples for analysis
- DNA binding dyes
- Glow Lab Dilution Buffers
- See resources below to download the teacher's guide and student guide for this lab. Contact team @ Southern Biological for answers key.
- This kit provides DNA samples, DNA ladder and gel loading dye only. To perform this lab, you will require electrophoresis equipment; such as electrophoresis chamber, agarose, TBE buffer and Midori green gel stain. Our electrophoresis starter kit contains the hardware and reagents required.
- For best results, we recommend running your gels in the blueGel electrophoresis chamber. If you do not have a blueGel electrophoresis unit with built-in illuminator, we have a transilluminator onto which you can place your gels to visualise your results immediately.
- These DNA samples require refrigerated storage (4 degrees), which will keep them stable for 6 months.