Description: This teacher demonstration kit contains fully illustrated instructions, one set of apparatus (see below) and sufficient materials for two demonstrations.
Carolina Biological Exploring Electrophoresis of DNA Kits.
Students separate mixtures of pre-cut DNA fragments using samples of lambda DNA:
- cut with EcoRI
- cut with HindIII
to effectively demonstrate the action of restriction enzymes and the principles of electrophoresis. Gels can be run in 3.5 hours using three 9V batteries or overnight with one battery. Apparatus and materials are included.
Apparatus consists of a gel chamber and comb, micro-pipettes and connecting cables.
Materials included are:
- Agarose and concentrated TBE buffer
- Ready-to-use pre-digested DNA samples, stable at room temperature.
- Carolina BLU® DNA stain
- Pipette tips.
- Carbon fibre electrodes.
The following items are required but not included:
- 9 volt batteries or equivalent DC power supply. The use of mains power is not recommended for this apparatus.
Notes: Also available:
G2.98CK - Classroom Activity Kit
The classroom activity kit contains fully illustrated instructions, five sets of apparatus (see above) and sufficient materials for ten experiments.
G2.98CK-RK - Classroom Refill Kit
The classroom refill kit contains replacement materials that can be used with the apparatus in G2.98 or G2.98CK. There are sufficient materials for ten experiments.
G2.98CK-DNA - Classroom DNA refill kit
The DNA refill kit contains only DNA replacements, sufficient for ten experiments
If you are not going to use the DNA within 30 days we recommend that the DNA samples be kept in the freezer until such time as you are ready to use them.