Products/Biotechnology/Kits and Experiments

SKU: KT-1001-03

miniPCR Genes in Space Food Safety Lab: Mars Colony at Risk!

AU$175.00 ex GST

T

n this 120-minute miniPCR Learning Lab, you will help the International Space Agency control an outbreak of pathogenic bacteria in space food bound for Mars! Learn how to use miniPCR(the PCR machine aboard the International Space Station), restriction analysis, and DNA gel electrophoresis to characterize bacterial strains and protect the health of astronauts.

This applied biotechnology investigation addresses a foodborne illness outbreak through the same real-world methodologies that public health and surveillance authorities use here on Earth. You’ll learn molecular microbiology techniques relevant to the STEM job market, and engage in a real-world problem with huge public health and economic impact.

To learn more about miniPCR aboard the International Space Station, watch this video or visit www.genesinspace.org.

This miniPCR Learning Lab utilizes the following molecular techniques:

  • Micropipetting
  • PCR (polymerase chain reaction) amplification of bacterial genes
  • Restriction digest, RFLP analysis
  • DNA Gel electrophoresis, staining, and visualization
  • Highlights real-world applications of molecular microbiology in public health.

The miniPCR Food Safety Lab kit contains reagents for 8 lab groups of 4 students:

  • 2X EZ PCR Master Mix, Load-Ready
  • 3X Food Safety Lab Primer Mix
  • 4 DNA samples to investigate, including controls
  • Restriction enzyme XmnI
  • 100 bp DNA ladder, Load-Ready
  • Reagents are compatible with miniPCR™blueGel™ or other standard PCR and DNA electrophoresis systems

 

Suggested skill level: Intended for any student seeking familiarity with PCR, DNA gel electrophoresis, environmental microbiology and antibiotic resistance, from middle school to college.

Please note:

  • These reagents require frozen storage.
  • Microtubes and electrophoresis reagents sold separately
  • None of the materials provided in the Food Safety laboratory kit pose a health or food safety hazard. At no point are pathogenic bacteria used; references to “pathogenic” and “non-pathogenic” strains or DNA samples are used only to recreate a simulated Food Safety investigation scenario.

 

Free miniPCR Learning Lab resources:

Contact team@ Southern Biological for answers key.

 

 

 

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