Students are presented with a fictional family’s medical history and must work to make a genetic diagnosis. The family depicted in this lab, the Robinson family, has two children, one of whom has had an initial test result indicating possible sickle cell disease.
This hands-on lab activity exposes students to the fundamental principles of DNA analysis comparing patterns of cleaved DNA by gel electrophoresis. Students will gain familiarity with DNA separation techniques and engage in determining DNA fragment size to obtain a genetic diagnosis.
- Techniques utilised: DNA gel electrophoresis.
- Time required: can be completed in one 45 min. class period
- Reagents needed: ‘Sickle Cell Genetics Lab’ reagents kit (included in this product), electrophoresis reagents (available separately - see below)
Suggested skill level: Intended for any student seeking familiarity with DNA gel electrophoresis, genetic disease and diagnosis, from high school to university.
The Sickle Cell Genetics Lab kit contains reagents for 8 lab groups of up to 5 students each (40 students):
- 4 DNA samples for fingerprinting analysis
- 1 tube of DNA molecular weight marker (DNA Ladder)
- Reagents are compatible with blueGel™ or other standard DNA electrophoresis systems
NOTE: The DNA samples in this kit are formulated for use with Midori green gel stain. For best results, we recommend running your gels in the blueGel electrophoresis chamber (G40.04). For use with other electrophoresis chambers, you will need a blue light transilluminator (e.g. G40.76) to visualise the results
These reagents require frozen storage.
Free miniPCR Learning Lab™ downloads:
- Sickle Cell Genetics Lab Instructor’s Guide
- Sickle Cell Genetics Lab Student’s Guide
- Sickle Cell Genetics Lab Classroom Slides
Contact team@ Southern Biological for answers key.
- Our electrophoresis starter kit contains the hardware and reagents required
- Hardware and reagents can also be purchased separately:
- If you do not have a blueGel electrophoresis unit, no problem! We have a transilluminator onto which you can place your gels as soon as they are done (as seen by the leading and trailing dyes) to visualise your results immediately.