Description: Separate and analyse amino acids using thin layer chromatography with this guided-inquiry kit.
Students study 3 amino acids, then identify the components of an unknown mixture of amino acids using observations of the chromatograms and Rf factors.
Amino acids: Alanine. Phenylalanine. Glycine.
Kit includes:
Materials are sufficient for 10 groups of students.
| Components | Qty | Included? |
|---|---|---|
| Ninhydrin, 0.1%, 10 mL | 1 | Included |
| 1:1 Butanol/Acetone, 30 mL | 1 | Included |
| 3 M Hydrochloric Acid, 30 mL | 1 | Included |
| 2-Propanol, 100%, 30 mL | 1 | Included |
| Glacial Acetic Acid, 15 mL | 1 | Included |
| Amino Acid Set (1g each) | 1 | Included |
| Mini Spoon | 3 | Included |
| Forceps | 10 | Included |
| Pipets | 5 | Included |
| Thin Layer Chromatography Plates | 12 | Included |
| Capillary Tube Vial | 1 | Included |
| Chromatography Chambers with Lids | 12 | Included |
| Digital Resource Card | 1 | Included |
| Teacher's Manual with Reproducible Student Guide | 1 | Included |
Needed but not included:
- 10 Pencils
- 10 Metric Rulers
- 4 Flasks, 50 mL
- 1 Flask, 100 mL
- 1 Graduated Cylinder, 100 mL (or smaller)
- Paper Towels
- Disposable Gloves
- Fume Hood
- Lab Oven or Hair Dryer (optional)
