Trypsin is supplied as powder that, in solution, acts to break down protein into its constituent amino acids. The source is mammalian (porcine).
Although trypsin will act on many different forms of protein, we have found its action on casein in low-fat milk allows good observations to be made. By using a light meter or colorimeter connected to a datalogger, you can plot the different reaction rates as conditions such as temperature and pH are varied.
Suggested experimental protocol (new window, 0.1M, pdf format)
Results of datalogging experiment - change in light transmittance of low fat milk treated with trypsin at different temperatures (new window, 0.1M, pdf format)
Enzyme FAQ (new window)
Enzyme overview (new window, 0.1M, pdf format)
Before ordering this product please review the relevant Safety Data Sheets (SDS) as part of a general risk assessment.
Enzyme SDS - Generic Enzyme Safety Data Sheet (new window, pdf format)