Description: Tried and tested Plant Cloning Investigation based on SAPS experiment protocol
In this investigation, students use plant tissue from a cauliflower to grow a genetically identical plant (clone). Cauliflower is the ideal plant for students to work with as it is accessible, fast growing, robust enough to withstand handling and the meristematic tissue from (the tips of the white curd) is easy to isolate. The techniques used in this investigation are adapted from those developed for plant scientists in the field.
Key Info and Benefits:
- The techniques used in this investigation are adapted from those developed for plant scientists in the field.
- Students are tasked with cutting small cauliflower florets into smaller explants and sterilising them using Sodium Dichloroisocyanurate (SDICN).
- The explants are then planted in agar plant growth media where students will observe their growth over a period of three weeks.
- After three weeks the cauliflower callus tissue develops small shoots, providing students with their own cauliflower clone.
- In this investigation, students gain practical experience in plant tissue culturing and gain a deeper understanding of plant cells and genetics.
Kit Includes: This kit contains enough materials for 8 lab stations allowing up to 32 student participation.
- 1 x 4.4g Murashige and Skoog Medium
- 1 x Kinetin 1g
- 1 x 50g Plain Agar powder
- 10 x Disposable Scalpels
- 10 x Sterile Petri Dishes
- 4 x Milton Tablets (SDICN)
- 10x Plastic Forceps
- 50 x Sterile Culture Vials 30ml