These products are supplied as 50uL aliquots pre-mixed with loading buffer and loading dye that are ready to load straight onto an agarose gel. At the recommended volume of 10uL per well, there is sufficient material for five runs. The high stability of these products means you can store unused material for up to 4 weeks in a refrigerator, or up to 6 months in a freezer.
G42.60 DNA Molecular Weight Marker, λDNA/HindIII, 50uL
Lambda phage DNA is produced in E.coli. After purification, the double stranded DNA (48,502 base pairs) is digested with the restriction enzyme HindIII to yield 8 fragments ranging in size from 23,130 to 125 base pairs. Mass of DNA = 10ug in 50uL of loading buffer/dye.
- Due to the larger size of the DNA fragments in these samples, use a 0.8% agarose gel in TBE buffer