This practical involves transforming bacteria (E. coli) with a gene from Aequorea victoria; a bioluminescent jellyfish. The green fluorescent protein (GFP) of the Aequorea victoria will be expressed in the bacteria; causing them to glow bright green
under UV light. Using bacterial plasmid-based genetic transformation, students will acquire the tools to transform E. coli bacteria to express new genetic information employing a plasmid system and applying mathematical routines to determine transformation efficiency.
This kit contains refill of consumables for BT1.00
Included in Kit:
24 x LB Agar Plain Plates (Perishable - refrigerate)
16 X LB Agar Ampicillin Plates (Perishable - refrigerate)
1 x E.coli MM294, in LB broth (Perishable - refrigerate if keeping for more than two weeks)
1x pGreen plasmid 100μL (Perishable - keep in freezer or for up to two weeks in the fridge)