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G42.90 - Dyes for electrophoresis

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Product Description

Your students will gain experience with the principles and practice of gel electrophoresis without the extra time and expense of running DNA. This product consists of prepared solutions of four dyes in water/glycerol:

  • Xylene cyanol
  • Orange G
  • Bromophenol blue
  • Methyl green

Due to differing polarity, load these dyes into the central well of the gel. The first three are negatively charged and migrate to the positive electrode, but Methyl Green carries a positive charge and migrates to the negative electrode. Glycerol in the solvent increases the density of the solutions so they sink into the wells and behave more like micro-samples of DNA mixed with buffered loading dye.

Since the dyes are visible in the gel, your students will observe the entire separation process as it unfolds, and no extra staining step is required. Suggested activities include deducing the components of dye mixtures and comparing the rate of migration at different voltage settings.

Volume supplied = approx 450uL of each dye solution (0.2% w/v), plus two vials containing different dye mixtures (approx 450uL each).



Click here to download a SDS. Please use this SDS as part of a general risk assessment. 

Other Details

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Product Videos

Gel Electrophoresis of Dyes (00:20)
The electrophoresis of polarised dyes allows an alternative to the electrophoresis of DNA in the classroom. This movie depicts the electrophoresis of a gel containing some basic dyes and two samples containing dye mixtures. The process allows the identification of the individual dyes contained in the mixed sample (simplified forensic example).
  • Gel Electropho...
    The electrophoresis of polarised dyes allows an alternative to...