Bacteria that produce light are very common in the ocean.
This practical involves transforming bacteria (E. coli) genes that are involved in the production of light (also known as lux genes). These genes have been removed from V. fischeri and placed in a plasmid, pVIB. TBacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.
This kit contains enough perishable only materials for 8 lab stations allowing up to 32 student participation.
Included in kit
1 x E.coli MM294, LB agar slope
1 x P Vib Plasmid, 100μL
2 x Ampicillin powder, 30mg
Contemplate Kit SKU: BT2.00