Bacteria that produce light are very common in the ocean.
This practical involves transforming bacteria (E. coli) genes that are involved in the production of light (also known as lux genes). These genes have been removed from V. fischeri and placed in a plasmid, pVIB. TBacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.
This kit contains enough materials for 8 lab stations allowing up to 32 student participation.
Included in Kit
16 x Transformation tube, 13mL, sterile
80 x Transfer pipette, 1mL, graduated, sterile
8 x CaCl2, 50mM, sterile, 10mL
16 x Inoculation loop, sterile, disposable
8 x Luria broth, sterile, 10mL
32 x Inoculation spreader, sterile, disposable
2 x Ampicillin powder, 30mg in vial (Perishable)
1 x Water, sterile, 20mL
10 x LB agar, 100mL (Melt and Pour)
40 x Petri dishes, sterile
1 x E.coli MM294, LB agar slope (Perishable)
1 x P Vib Plasmid, 100μL (Perishable)
Perishable Refills also available SKU: BT2.10