Loading... Please wait...

Categories

Newsletter Archive

To subscribe to our e-newsletter

Click Here

Download Price List

To view downloadable price list...

Click Here

MC23.92S - Urease, 10g

Price:
AU$61.30
SKU:
MC23.92S
Availability:
Perishable. Goods that are living or perishable. Despatched on Mon, Tues and Wed only. Collection available Mon to Fri after 10am


Product Description

There are many different forms of urease in nature, but all have the common function of catalysing the breakdown of urea into carbon dioxide and ammonia.  In plants, this is usually a means to access the nitrogen in urea, but in many bacteria, it is thought to be a protective enzyme.  In such cases, the bacteria are able to produce ammonia to neutralise acids in their immediate environment.  The presence of urease in soil bacteria can be a problem in agriculture if the bacteria are able to break down urea based fertilisers before plants have been able to benefit.

Download

Suggested experimental protocol (new window, 0.1M, pdf format)

Enzyme FAQ (new window)

Enzyme overview (new window, 0.1M, pdf format)

Please Note:

Before ordering this product please review the relevant Safety Data Sheets (SDS) as part of a general risk assessment.

Enzyme SDS -  Generic Enzyme Safety Data Sheet (new window, pdf format)    

 

Other Details

Unit Size:
10g

Product Videos

UreaseReaction (01:35)
Prepare a urease solution of approximately 1% by adding 1g of urease to 100mL of distilled water in a flask. Stopper and agitate for 5 minutes, then allow the sediment to settle. Pour off the clear supernatant liquid. This will be your urease solution. Place 5mL of 1% urea solution into a test tube and add 10 drops of universal indicator. Then add 3mL of urease solution to the mixture in the test tube. As a control, repeat this process using 3mL of distilled water instead of urease solution. Note the change in colour of the solution as the enzyme reaction causes the pH to increase due to the formation of ammonia.
  • UreaseReaction
    Prepare a urease solution of approximately 1% by adding 1g of ...