This practical involves transforming bacteria (E. coli) with a gene from Aequorea victoria; a bioluminescent jellyfish. The green fluorescent protein (GFP) of the Aequorea victoria will be expressed in the bacteria; causing them to glow bright green
under UV light. Using bacterial plasmid-based genetic transformation, students will acquire the tools to transform E. coli bacteria to express new genetic information employing a plasmid system and applying mathematical routines to determine transformation
This kit contains enough perishable only materials for 8 lab stations allowing up to 32 student participation.
Included in kit
1 x E.coli MM294, LB agar slope
1 x pGreen Plasmid 100μL
2 x Ampicillin powder, 30mg
Contemplate Kit SKU: BT1.00