Can you discover the conditions that affect the rate of enzyme catalyzed reactions? …And make them glow!
This lab allows students to investigate enzyme activity and the conditions that affect it. Under optimal conditions enzymes catalyze, or speed up the rate of, chemical reactions. In this lab, a substrate that is not fluorescent under basal conditions, will fluoresce under blue light when sugar molecules are removed through hydrolysis by the enzyme beta-galactosidase. Observing how brightly the solution fluoresces over time allows students to observe the reaction progress, and to quantify the effect of different factors on enzyme efficiency.
This Lab is structured as four inquiry-based investigations. In each investigation, students explore the effect of a different factor on enzyme reaction rate:
This lab provides enough reagents for eight lab groups to each pursue at least two of these investigations (32 students):
Required equipment (one per lab group)
Contact team@ Southern Biological for answers key.