These products are supplied as 50uL aliquots pre-mixed with loading buffer and loading dye that are ready to load straight onto an agarose gel.  At the recommended volume of 10uL per well, there is sufficient material for five runs.  The high stability of these products means you can store unused material for up to 4 weeks in a refrigerator, or up to 6 months in a freezer.

G42.20

DNA Molecular Weight Ladder, 500 base pairs, 50uL

At least 13 DNA fragments in exact increments of 500 base pairs, with a stronger band at 5,000 base pairs.  Mass of DNA = 9ug in 50uL of loading buffer/dye.

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G42.30

DNA Molecular Weight Ladder, 1000 base pairs, 50uL

At least 13 DNA fragments in exact increments of 1000 base pairs, with a stronger band at 5,000 base pairs.  Mass of DNA = 7ug in 50uL of loading buffer/dye.

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G42.40

DNA Molecular Weight Ladder, 2500 base pairs, 50uL

At least 10 DNA fragments in exact increments of 2500 base pairs, with a stronger band at 10,000 base pairs.  Mass of DNA = 5ug in 50uL of loading buffer/dye.

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G42.51

DNA Molecular Weight Marker,  λDNA/BstEII, 50uL

Lambda phage DNA is produced in E.Coli. After purification, the double stranded DNA (48,502 base pairs) is digested with the restriction enzyme BstEII to yield 14 fragments ranging in size from 8,454 to 117 base pairs. Mass of DNA = 10ug in 50 uL of loading buffer / dye. 

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G42.60

DNA Molecular Weight Marker, λDNA/HindIII, 50uL

Lambda phage DNA is produced in E.coli.  After purification, the double stranded DNA (48,502 base pairs) is digested with the restriction enzyme HindIII to yield 8 fragments ranging in size from 23,130 to 125 base pairs.  Mass of DNA = 10ug in 50uL of loading buffer/dye.

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Simulated forensic exercise - provide students with two or more DNA samples plus one duplicate sample.  Treat each sample as having been collected from a crime scene and from two suspects.  Run an electrophoresis experiment to determine which of the two suspects is the "guilty party".

Measure DNA fragment sizes - run a sample of G42.51 and/or G42.60 using G42.30 as a molecular weight ladder.  Use the known fragment sizes of G42.30 to estimate the fragment sizes of G42.51 and G42.60.

Construct plots - provide students with information about the number of base pairs in a limited number of bands, and have them plot log (no. base pairs) versus distance in order to calculate the number of base pairs in the other bands.

Compare action of restriction enzymes - compare the bands that result from running G42.51 beside G42.60. Have students explain why the same DNA ( λDNA in each case) gives different banding patterns after being cut with different restriction enzymes.